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KMID : 0355420120360020091
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Journal of Korean Academy of Oral Health
2012 Volume.36 No. 2 p.91 ~ p.96
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Comparison of mRNA expression of gtf genes and adhesive ability of xylitol-sensitive and-resistant Streptococcus mutans by xylitol-treated concentrations
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Im Sang-Uk
An Seo-Young
Choi Youn-Hee
Song Keun-Bae
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Abstract
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Objectives: Xylitol is an effective anticarious natural sugar substitute, by inhibiting the virulence of Streptococcus
mutans (S. mutans ). However, long-term xylitol consumption leads to an emergence of the xylitol-resistant (XR) strains. This study aimed to confirm the general characteristics, mRNA expression of gtf genes, and adhesive ability of the xylitol-sensitive (XS) and XR S. mutans , by xylitol-treated concentrations.
Methods: S. mutans KCTC3065 was maintained in TYE medium, containing 0.4% glucose with 1% xylitol for 30 days at 37oC, 10% CO2 to form XR strain and the same procedures, without xylitol, were repeated for the formation of XS S. mutans . Both XS and XR were cultured by xylitol-treated concentrations (0%, 0.1% and 1%), then, general
characteristics, such as growth and acid production, mRNA expression of gtf genes and adhesive ability were analyzed.
Results: Xylitol reduced the cell growth of XS S. mutans in a dose-dependent manner, but did not reduce the XR. Xylitol inhibited acid production of XS in a dose-dependent manner. However, it did not inhibit that of XR. Xylitol reduced the gtfB and gtfD mRNA expression of the XS S. mutans , which the genes synthesized soluble and insoluble extracellular polysaccharides, but not reduced that of the XR. By a microtiter plate assay, biofilm formation was more reduced in the XR strains, which means biofilm’s adhesive ability of XR S. mutans was lower than that of the XS.
Conclusions: These results indicate that a lower level of adhesive ability for XR S. mutans is related with mRNA expression level of gtf genes, which suggested that the XR strains may be less cariogenic than that of the XS.
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KEYWORD
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Adhesive ability, Glucosyltransferase, Streptococcus mutans, Xylitol-resistant, Xylitol-sensitive
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